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Protein Synthesis I

  • Remember that:
    • each chromosome is made up of a long, super-coiled molecule of DNA
    • each molecule is divided into thousands of shorter sections called genes
    • a gene is a small part of the DNA strand and codes for a protein.
    • the length of DNA making up a particular gene carries the information needed to make a particular protein
    • inside every cell, there are thousands of different chemical reactions taking place.

DNA

  • Enzymes control all the chemical reactions that occur inside our body.
  • It is important to remember that all enzymes are proteins.
    • Since DNA codes for proteins, it determines:
      • which enzymes are produced in each cell,
      • and therefore which chemical reactions take place inside cells.
  • The structure of DNA contains bases. The information is found in the sequence of bases along the length of DNA.
    • The sequence of bases in a gene determines the sequence of amino acids used to make a specific protein.
  • DNA also controls cell function by controlling the production of proteins, including enzymes, membrane carriers, and receptors for neurotransmitters.
  • DNA carries information to build proteins from amino acids. The information moves from the DNA to the site of protein synthesis in the ribosomes via a kind of messenger molecule.
    • This messenger molecule is called the RNA. The name given is messenger RNA (mRNA).
    • The role of mRNA is to carry a copy of the base sequence on DNA out of the nucleus to the ribosomes in the cytoplasm where protein synthesis occurs.

The genetic code

  • A, T, C and G are the four different bases found in DNA.
  • Each amino acid is coded for, by a sequence of three of these bases on DNA.
    • There are about 20 different amino acids and each is coded for by a different base triplet on DNA.
    • Different sequences of amino acids give different shapes to protein molecules.
  • When mRNA copies the base sequence on DNA, it does so by the rule of base pairing.
    • The rule of base pairing states that specific nitrogenous bases in DNA and RNA always pair up with each other through hydrogen bonds.
      • In terms of DNA base pairing rules, A is always paired with T and C is always paired with G.
      • mRNA does not have the base T, therefore it is replaced with base U (uracil). A on DNA will code for U on mRNA.
  • The coded information on mRNA is used to assemble amino acids in the correct sequence for each protein.
    • Scroll down for what the syllabus says for how a protein is made.
    • The mRNA arrives at the ribosomes from the nucleus.
    • The mRNA strand contains the base triplets for each particular amino acid in the protein.
    • The mRNA then passes through the ribosomes and each ribosome assembles amino acids into protein molecules.
    • The amino acids bond together forming a long chain, the protein.
    • The specific order of amino acids is determined by the sequence of bases in the mRNA and each amino acid is joined to the next one by a peptide bond.
  • All body cells in an organism contain the same genes. However, in a particular cell, many genes may not be expressed because the cell makes only the specific proteins it needs.

Syllabus definition for protein creation

  • Explain how a protein is made, limited to:
    • the gene coding for the protein remains in the nucleus
    • messenger RNA (mRNA) is a copy of a gene
    • mRNA molecules are made in the nucleus and move to the cytoplasm
    • the mRNA passes through ribosomes
    • the ribosome assembles amino acids into protein molecules
    • the specific sequence of amino acids is determined by the sequence of bases in the mRNA.